Effects of Ducrosia anethifolia essential oil as diet supplement on immune parameters of rainbow trout (Oncorhynchus mykiss)
Authors
1 Department of Natural Resources and Environmental Engineering, Faculty of Agriculture, Shiraz University, Shiraz
2 Department of Natural Recourses and Environmental Engineering, Faculty of Agriculture, Shiraz University, Shiraz
Abstract
Recently immune stimulants have been widely used as substitution of antibiotics to prevent diseases in aquaculture. In this study, the effects of Ducrosia anethifolia essential oil on growth and immune responses of rainbow trout (Oncorhynchus mykiss) were evaluated. The essential oil was mixed with sunflower oil at different concentrations (0.001, 0.01 and 0.1 %), added to diets and fish were fed with these diets for 40 days. white blood cell count, serum lysozyme, bactericidal and respiratory burst activity were measured every ten days. The mean of white blood cells (WBC) in 0.01 and 0.1 groups were higher than those in 0.001 and control groups. The mean of serum bactericidal activity showed no significant difference among different treatments but the fourth treatment on day 10 and the control group on day 30 showed the highest serum bactericidal activities. The highest and lowest mean of respiratory burst was observed in 0.01 and 0.001 treatments, respectively.
Keywords
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